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iLine F

Digital holographic microscopy for suspension cell culture

Manufacturer:

The iLine F microscope uses a unique concept for in-line, non-invasive and label-free suspension cell counting, offering its users an unmatched experience when combined with a bioreactor. Continuously tracking cells in real-time with high accuracy, the system has been designed as a particularly efficient tool for deep investigation of underlying data. Fast and accurate, the algorithms automatically discriminate living from dead cells, count clusters and eliminate debris. 


The microscope captures holograms of the cells travelling through the BioConnect disposable fluidics system, which connects the microscope to the bioreactor. The fluidics system is activated via a unique membrane pump, applying the principle of a heart and proven not to affect cells, while the closed loop set-up ensures sterility and continuous viable cell counting and monitoring without sample consumption. 
Transfer of OVIZIO's technology from one site to another becomes very convenient (not operator dependent) and much less time consuming.

The system is straightforwardly scalable, and can be used from R&D to process development and production.

Abstract:

  • No sampling, no staining, no toxic waste
  • Full traceability at single cell level
  • Discrimination between dead and living cells
  • Cluster's information without disruption
  • Continuous and real-time data acquisition
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Contact

Stefan Mahr
Phone : +49 2223 9192 142
Email : sma@il-biosystems.de

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