Key features
- 8-channel system
- 384-well plate format
- Measure 1 – 456 samples per run
- Wide range of biosensors with improved surface chemistry
- Automated biosensor loading and regeneration
- Intuitive software with integrated assay setup, data acquisition and analysis
Sales in
Austria, Belgium, Germany, Luxembourg, Sweden, Switzerland, The Netherlands
GatorPlus – Biolayer Interferometry (BLI) System
The GatorPlus from Gator Bio is a label-free analysis instrument based on the biolayer interferometry (BLI) technology. It´s main applications are biomolecular quantitation, determination of intermolecular binding kinetics and epitope binning. This 8-channel system can quantify 8 samples in 60 second and 384 samples in less than 120 minutes. The real-time detection of binding kinetics allows for the determination of kon, koff and Kd of up to 192 samples in one experiment. A double thermal insulation of the optical bench leads to best in-class baseline stability, which improves the sensitivity while measuring long off-rates of tight binding interactions. The 384-well plate format enables 16×16 epitope binning experiments in the classical sandwich or tandem assay. The GatorPlus is a fluidic-free benchtop instrument that is robust enough to handle everything from purified samples to crude extracts or serum.
The integrated software allows for intuitive assay setup, data acquisition and analysis. Gator Bio also offers a new generation of biosensors with improved surface chemistry for enhanced sensitivity, binding capacity, robustness and regenerative capability. A wide choice of pre-coated biosensors allows for the quantitation and kinetic analysis of proteins, antibodies, peptides, nucleic acids, aptamers, liposomes, AAV and small molecules. The GatorPlus is our advanced BLI instrument that provides a powerful and versatile tool to scientists from drug discovery to therapeutics manufacturing QC.
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- Regenerable biosensors to save time and cost per assay
- Best in-class baseline stability to measure long off-rates of tight binders
- Quantify 384 samples in under 120 minutes
- Determine kon, koff and Kd of up to 192 samples in one run
- 16×16 Epitope binning
- Can be used with crude extracts, serum or plasma
- Supports 21 CFR part 11 (GMP, GLP)
Discover the next generation of BLI technology!
Gator Bio 96-well flat plate
The Gator Bio® BLI 96-Flat Plate is a black polypropylene 96-well flat-bottom plate that meets the Standard Society for Biomolecular Screening (SBS) specifications. It is designed for use in Bio-Layer Interferometry (BLI) experiments that measures biomolecular interactions of proteins, peptides, small molecules, and viruses. The BLI 96-Flat plate can be utilized on Gator Bio® instruments in an array of applications, as well as other BLI platforms that accommodate a 96-well microplate format.
Gator Bio® BLI 96-well plates are available by us – please feel free to ask us!
Consumables
Working with Antibodies
| Probe | Dynamic Range | Regeneration |
| Protein A | 0.02 – 2000 µg/ml | Yes |
| Protein G | 0.02 – 2000 µg/ml | Yes |
| Protein L | 0.02 – 2000 µg/ml | Yes |
| Anti-Human IgG Fc Gen II | 0.3 – 6000 µg/ml | Yes |
| Anti-Human FAB | 0.3 – 3000 µg/ml | Yes |
| Anti-Mouse IgG Fc | 0.05 – 100 µg/ml | Yes |
Working with AAV
| Probe | Serotypes | Dynamic Range | Regeneration |
| AAVX | AAV 1-8 and 10 | 1 x 109 – 1 x 1013 vp/mL | Yes |
| AAV9 | AAV 9 | 1 x 109 – 1 x 1013 vp/mL | Yes |
| High sensitivity AAV | AAV 1-8 | 5 x 106 – 5 x 1010 vp/mL | No |
Working with other biomolecules
| Probe | Regeneration |
| Streptavidin | No |
| Streptavidin FLEX | Yes |
| Strep-Tactin XT | Yes |
| Aminopropylsilane | No |
| Amine Reactive | No |
| Anti-His | Yes |
| Ni-NTA | Yes |
| Small Molecule Analysis | No |
Downloads
Gator bio AAV
Free analysis with BLI
Brochure
Application note - Gator Bio AAV Quantitation
Technical data
| Detection technology: | Biolayer Interferometry |
| Analysis types: | Quantitation, Yes/No Binding, Kinetics, Affinity |
| Sample types: | Proteins, Antibodies, Peptides, Nucleid Acids, Liposomes, Small Molecules, AAV |
| Number of Channels: | 8 |
| Data Aquisition rate: | 2 Hz, 5 Hz, 10 Hz |
| Plate format: | 1 x 96- or 1 x 384-well plate & 1x 96-well max plate |
| Max samples per run: | 456 |
| Quantitation throughput: | 384 samples in less than 2 hours |
| Kinetics per run: | 192 |
| Epitope binning: | 16 x 16 |
| Minimum sample volume: | 40 µl |
| Baseline noise: | ≤ 4 pm (RMS) |
| Baseline drift: | ≤ 0.1 nm/hour |
| Association rate kon: | 101 – 107 M-1 s-1 |
| Dissociation rate koff: | 10-6 – 10-1 s-1 |
| Affinity constant Kd: | 10 pm – 1 mM |
| Orbitaler Fluss: | 100 – 2000 rpm |
| Temperature: | Ambient to 40°C |
| Dimensions: | 63 cm x 73 cm x 44 cm (H x W x D) |
| Weight: | 50 kg |
| Electrical: | 110-220 V |
| Power consumption: | 200 W |
| Safety Standards: | CE, CSA, FCC |
