Key features
- 8-channel system
- 96-well format
- Measure 1 – 168 samples per run
- Wide range of biosensors with improved surface chemistry
- Automated biosensor loading and regeneration
- Intuitive software with integrated assay setup, data acquisition and analysis
Sales in
Austria, Belgium, Denmark, France, Germany, Ireland, Luxembourg, Sweden, Switzerland, The Netherlands, United Kingdom
GatorPrime – Biolayer Interferometry (BLI) system
The GatorPrime from Gator Bio is a label-free analysis instrument based on the biolayer interferometry (BLI) technology. It´s main applications are biomolecular quantitation, determination of intermolecular binding kinetics and epitope binning. This 8-channel system can quantify 8 samples in 60 second and 96 samples in less than 30 minutes. The real-time detection of binding kinetics allows for the determination of kon, koff and Kd of up to 72 samples in one experiment. An extra thermal insulation of the optical bench leads to improved baseline stability, which increases the sensitivity while measuring long off-rates of tight binding interactions. The 96-well plate format enables 10×10 epitope binning experiments in the classical sandwich or tandem assay. The GatorPrime is a fluidic-free benchtop instrument that is robust enough to handle everything from purified samples to crude extracts or serum. The integrated software allows for intuitive assay setup, data acquisition and analysis. Gator Bio also offers a new generation of biosensors with improved surface chemistry for enhanced sensitivity, binding capacity, robustness and regenerative capability.
A wide choice of pre-coated biosensors allows for the quantitation and kinetic analysis of proteins, antibodies, peptides, nucleic acids, aptamers, liposomes, AAV and small molecules. The GatorPrime is our entry level BLI instrument that provides a powerful and versatile tool to scientists from drug discovery to therapeutics manufacturing QC and in academia.
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- Regenerable biosensors to save time and cost per assay
- Improved baseline stability to measure long off-rates of tight binders
- Quantify 96 samples in under 30 minutes
- Determine kon, koff and Kd of up to 72 samples in one run
- 10×10 Epitope binning
- Can be used with crude extracts, serum or plasma
- Supports 21 CFR part 11 (GMP, GLP)
Discover the next generation of BLI technology!
Gator Bio 96-well flat plates
The Gator Bio® BLI 96-Flat Plate is a black polypropylene 96-well flat-bottom plate that meets the Standard Society for Biomolecular Screening (SBS) specifications. It is designed for use in Bio-Layer Interferometry (BLI) experiments that measures biomolecular interactions of proteins, peptides, small molecules, and viruses. The BLI 96-Flat plate can be utilized on Gator Bio® instruments in an array of applications, as well as other BLI platforms that accommodate a 96-well microplate format.
Gator Bio® BLI 96-well plates are available by us – please feel free to ask us!
Consumables
Working with Antibodies
Probe | Dynamic Range | Regeneration |
Protein A | 0.02 – 2000 µg/ml | Yes |
Protein G | 0.02 – 2000 µg/ml | Yes |
Protein L | 0.02 – 2000 µg/ml | Yes |
Anti-Human IgG Fc Gen II | 0.3 – 6000 µg/ml | Yes |
Anti-Human FAB | 0.3 – 3000 µg/ml | Yes |
Anti-Mouse IgG Fc | 0.05 – 100 µg/ml | Yes |
Working with AAV
Probe | Serotypes | Dynamic Range | Regeneration |
AAVX | AAV 1-8 and 10 | 1 x 109 – 1 x 1013 vp/mL | Yes |
AAV9 | AAV 9 | 1 x 109 – 1 x 1013 vp/mL | Yes |
High sensitivity AAV | AAV 1-8 | 5 x 106 – 5 x 1010 vp/mL | No |
Working with other biomolecules
Probe | Regeneration |
Streptavidin | No |
Streptavidin FLEX | Yes |
Strep-Tactin XT | Yes |
Aminopropylsilane | No |
Amine Reactive | No |
Anti-His | Yes |
Ni-NTA | Yes |
Small Molecule Analysis | No |
Downloads
Brochure
Application note - Gator Bio AAV Quantitation
Flyer
Application Note - Gator Bio Biotinylation Protocol
E-book - Gator Bio Antibody Solutions
Technical data
GENERAL | |
Detection: | Biolayer interferometry |
Number of channels: | 8 |
Data collection rate : | 2 Hz, 5 Hz, 10 Hz |
Orbital flow: | 0 – 1,500 rpm |
Dimensions: | 460 mm x 670 mm x 320 mm (H x W x D) |
Weight: | 30 kg |
Electrical: | 110 V – 220 V 5A |
Power: | 200 W |
KINETICS | |
Analysis time: | Real-time kinetic binding from 1 minute to 4 hours |
Association rate kon: | kon 101 – 106 M-1 /s |
Dissociation rate koff: | koff 10-6 – 10-1 /s |
Affinity constant KD: | KD 10 pM – 1 mM |
Binding curve baseline nois: | <3 pm (RMS) |
Binding curve baseline drift: | <40 pm/hour (RMS) |
ANALYSIS | |
Sample types : | Proteins, antibodies, peptides, DNA, RNA, liposomes, cells, viruses, etc. |
Work flow: | • 2 x 96-well plates, in tilted or flat position • Up to 8 samples in parallel • Up to 96 samples per 96-well plate |
Types of analysis: | • Yes or no binding • Relative and absolute concentration • Kinetics and affinity |
QUANTITATION | |
Analysis time IgG-Concentration: | • in 30 seconds for 8 samples • <10 minutes for 96 samples |
Linear range for Gator Protein A biosensor: | • 0,025 – 2.000 μg/mL |