Key features
- 32-channel system
- Quantitate 1152 samples in 142 minutes
- Wide range of high sensitivity biosensors
- Automation compatible
- Intuitive data acquisition and analysis software
Sales in
Austria, Belgium, Germany, Luxembourg, Switzerland, The Netherlands
GatorPro – Biolayer Interferometry (BLI) system for high throughput
The GatorPro from Gator Bio is a label-free analysis instrument based on the biolayer interferometry (BLI) technology. It´s main applications are biomolecular quantitation, determination of intermolecular binding kinetics and epitope binning. This 32-channel system can quantify 32 protein or AAV samples in 120 second or up to 1152 samples in 142 minutes. The real-time detection of binding kinetics allows for the determination of kon, koff and KD of up to 32 interaction pairs in parallel. Improved baseline stability allows for extra-long measurements of the dissociation step to precisely determine off-rates of tight binding interactions. The 3 x 384-well plate setup enables 32 x 32 epitope binning experiments in the classical sandwich or tandem assay and the automation compatibility allows for extra-long walk away times.
GatorPro is fluidics-free and robust enough to handle everything from purified samples to crude extracts or serum, which makes it easily integrated into every step of your process. The included software allows for intuitive assay setup, data acquisition and analysis that even unexperienced users can use easily. Additionally, Gator Bio also offers a new generation of biosensors with improved surface chemistry for enhanced sensitivity, binding capacity, robustness and regenerative capability.
A wide choice of pre-coated biosensors allows for the quantitation and kinetic analysis of proteins, antibodies, peptides, nucleic acids, aptamers, lipid nanoparticles, AAV and small molecules. The GatorPro is our most powerful BLI instrument that pairs high throughput with state-of-the-art sensitivity. This makes it a versatile tool for scientists from early drug discovery to therapeutics manufacturing QC.
• Regenerable biosensors to save time and cost per assay
• Improved baseline stability for prolonged off-rate measurements of tight binders
• Determine kon, koff and KD of up to 32 samples in parallel
• 32 x 32 Epitope binning
• Works with crude extracts, serum or plasma
• Supports 21 CFR part 11 (GMP, GLP)
Discover the next generation of BLI technology!
Gator Bio 96-well flat plates
The Gator Bio® BLI 96-Flat Plate is a black polypropylene 96-well flat-bottom plate that meets the Standard Society for Biomolecular Screening (SBS) specifications. It is designed for use in Bio-Layer Interferometry (BLI) experiments that measures biomolecular interactions of proteins, peptides, small molecules, and viruses. The BLI 96-Flat plate can be utilized on Gator Bio® instruments in an array of applications, as well as other BLI platforms that accommodate a 96-well microplate format.
Gator Bio® BLI 96-well plates are available by us – please feel free to ask us!
Consumables
Working with Antibodies
| Probe | Dynamic Range | Regeneration |
| Protein A | 0.02 – 2000 µg/ml | Yes |
| Protein G | 0.02 – 2000 µg/ml | Yes |
| Protein L | 0.02 – 2000 µg/ml | Yes |
| Anti-Human IgG Fc Gen II | 0.3 – 6000 µg/ml | Yes |
| Anti-Human FAB | 0.3 – 3000 µg/ml | Yes |
| Anti-Mouse IgG Fc | 0.05 – 100 µg/ml | Yes |
Working with AAV
| Probe | Serotypes | Dynamic Range | Regeneration |
| AAVX | AAV 1-8 and 10 | 1 x 109 – 1 x 1013 vp/mL | Yes |
| AAV9 | AAV 9 | 1 x 109 – 1 x 1013 vp/mL | Yes |
| High sensitivity AAV | AAV 1-8 | 5 x 106 – 5 x 1010 vp/mL | No |
Working with other biomolecules
| Probe | Regeneration |
| Streptavidin | No |
| Streptavidin FLEX | Yes |
| Strep-Tactin XT | Yes |
| Aminopropylsilane | No |
| Amine Reactive | No |
| Anti-His | Yes |
| Ni-NTA | Yes |
| Small Molecule Analysis | No |
Downloads
Application note - Gator Bio AAV Quantitation
Flyer
Application Note - Gator Bio Biotinylation Protocol
E-book - Gator Bio Antibody Solutions
Technical data
| GENERAL | |
| Detection: | Biolayer interferometry |
| Number of channels: | 32 |
| Data collection rate : | 2 Hz, 5 Hz, 10 Hz |
| Orbital flow: | 0 – 2,000 rpm |
| Dimensions: | 840 mm x 1140 mm x 770 mm (H x W x D) |
| Weight: | 220 kg |
| Electrical: | 110 V – 220 V 5A |
| Power: | 200 W |
| KINETICS | |
| Analysis time: | Real-time kinetic binding from 1 minute to 4 hours |
| Association rate kon: | kon 101 – 107 M-1 /s-1 |
| Dissociation rate koff: | koff 10-6 – 10-1 /s |
| Affinity constant KD: | KD 10 pM – 1 mM |
| Binding curve baseline nois: | <4 pm (RMS) |
| Binding curve baseline drift: | <0.1 nm/hour (RMS) |
| ANALYSIS | |
| Sample types : | Proteins, antibodies, peptides, DNA, RNA, liposomes, cells, viruses, etc. |
| Work flow: | • 3x 384 or 96 well plates and 1x 96 well max plate • Up to 32 samples in parallel |
| Types of analysis: | • Yes or no binding • Relative and absolute concentration • Kinetics and affinity |
| QUANTITATION | |
| Analysis time IgG-Concentration: | • in 120 seconds for 32 samples • 1152 samples in 142 minutes |
| Linear range for Gator Protein A biosensor: | • 0,025 – 2.000 μg/mL |
