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Protein purification workflows often fall into one of two approaches in a research environment or during the development. On one side are single-channel FPLC systems, known for their precision and essential for experiments requiring finely tuned linear gradients or for establishing which factors truly influence a separation. On the other side are high-throughput robotic platforms, valuable in small-scale candidate identification, particularly in microtiter plate formats.
The ProteinMaker was developed to bridge the gap between these two approaches. It combines the scale (volume flexibility and options), separation techniques and purity levels associated with small-scale FPLC systems with the speed and parallelisation of high-throughput approaches. Unlike either extreme, it maintains exceptional flexibility in sample volume, sample type, separation method and elution parameters. Its volumes are closer to single-channel FPLC systems, but it can cover the entire range of either platform. This combination makes it a true middle-ground solution for labs, especially in multi-sample environments, looking to accelerate protein purification without compromising on quality or adaptability.
A flexible and scalable solution
The ProteinMaker protein purification system is capable of purifying up to 24 samples at once. This dramatically reduces total purification time compared to single-channel FPLC systems that run samples sequentially. While some FPLC systems can automate sequential purification of several samples overnight as an example, they cannot match the throughput of the ProteinMaker in a single setup. For projects requiring many protein samples for screening or assay development, this speed is critical. One consideration, in addition, is that protein stability in the elution buffer and the timing of downstream characterisation still need to be carefully managed—and that will be easier if everything is processed at the same time on the ProteinMaker.
Each of the 24 channels operates independently but can also be grouped together in any configuration. This allows researchers to run different buffers, protocols, separation techniques or even entirely different sample types in parallel. For method development, upscaling and optimisation, this is invaluable. Testing multiple purification parameters simultaneously accelerates discovery and ensures the best conditions are identified early.
The ProteinMaker works with standard chromatography columns of 0.5 mL, 1 mL, 5 mL, or larger from any supplier. Among these, the 1 mL and 5 mL formats are the most commonly used. It can process volumes ranging from just a few millilitres to more than a litre, yielding milligram to multi-gram quantities. Using the same column types, flow rates, and protocols as single-column systems ensures results that are consistent, predictable, and scalable.
Running multiple columns in parallel also allows for rapid screening of different purification conditions. For example, a researcher can evaluate multiple wash buffers to improve host cell protein removal, or compare different resins such as Protein A and Protein G for a specific mouse IgG2a antibody. These experiments, which might take days on a single-channel system, can be completed in a single ProteinMaker run.
For applications that require fewer simultaneous purifications but the same flexible approach, the ProteinMaker chromatography system 6 channels offers a scaled-down option without compromising on capability.
How the ProteinMaker stands out
Compared to single-channel systems
The ProteinMaker revolutionizes protein purification by operating as a multi-channel, parallel processing system. This design allows you to accelerate your research and optimize your lab’s resources in ways single-channel systems cannot match.
The ProteinMaker streamlines entire workflows as a true walk-away automation. Its ability to process multiple samples and conditions simultaneously dramatically shortens method development timelines. Instead of running experiments sequentially, ranges of parameters can be explored in a parallel, unattended run, significantly reducing hands-on time and freeing you up for other critical tasks.
While speed is often critical, the ProteinMaker is engineered for flexibility, effortlessly accommodating variable sample volumes without the constraints of dedicated sample loops or the need for pre-loading. This high-throughput capability delivers the output of several single-channel systems from one compact instrument, saving valuable bench space and consolidating your purification efforts efficiently.
By being able to handle every standard run up to complex, multi-sample purifications, the ProteinMaker optimizes the use of all other laboratory equipment. It frees up traditional FPLC instruments to perform the single-run applications for which they are best suited. This ensures every instrument is used to its full potential, maximizing overall productivity and preventing bottlenecks.
Compared to fully automated robotic systems
The ProteinMaker is offering a unique combination of throughput, flexibility, and scalability that sets it apart from typical high-throughput robotics.
The ProteinMaker is built on standard, scalable chromatography principles. This is a key advantage, as it ensures the methods are directly transferable to larger FPLC production systems without costly and time-consuming redevelopment. The move from discovery to process development can be done confidently, avoiding the scale-up challenges often encountered with systems that rely on different separation technologies like magnetic beads or fixed resins.
The ProteinMaker goes beyond the limitations of screening and can produce the sample quantities actually needed for further process or drug development. While many robotic systems are optimized for sub-milligram yields, the ProteinMaker excels at mid-scale purification, capable of delivering up to 100 mg or more of protein. This provides ample, high-quality material for comprehensive downstream assays, detailed characterization, and further process optimization.
The ProteinMaker delivers the power of parallel processing in an accessible, user-friendly package. It combines an efficient scale and footprint with the robust experimental freedom of a larger FPLC operation. Complete control and flexibility over sample volumes, separation types, elution profiles, and protocol design is retained, all while benefiting from the speed and efficiency of a multi-channel, automated system.
Bridging the gap in protein purification
The ProteinMaker’s unique combination of throughput, flexibility and scalability makes it a valuable tool for a wide range of applications, from basic research to process development for manufacturing. It enables faster method optimisation, consistent scalability and efficient parallel workflows that single-channel and fully automated systems struggle to match. For labs that need both adaptability and productivity, it offers a practical solution that reduces bottlenecks and supports innovation at every stage of protein purification.
Ready to accelerate your protein purification workflow?
At I&L Biosystems, we help research and development teams find the right tools for their unique challenges. Whether you’re exploring the full capabilities of the ProteinMaker protein purification system or considering the more compact 6-channel configuration, our team is here to provide expert advice and support.
Get in touch with your local I&L Biosystems representative to discuss your application and discover how the ProteinMaker can transform your purification process.