Key features
- Captures Spatial Data, including cell morphology and marker localisation.
- Visualises Subcellular Structures, analysing spatial variations in brightfield, darkfield, and fluorescence images.
- Enhances Sorting Precision by integrating imaging features into gating strategies for accurate cell phenotype identification.
- Combines High-Throughput Sampling with Single-Cell Imaging, extracting extensive feature data from each cell.
Sales in
Austria, Belgium, Denmark, France, Germany, Ireland, Luxembourg, Sweden, The Netherlands
The VERLO™ Image-Guided Cell Sorter utilises patented microfluidic-based sorting, combining laser excitation with sensitive PMT detectors to isolate mammalian cells, microbes, and plant cells. A precise piezoelectric actuator directs cells into collection channels, enabling analysis and sorting in a disposable format, preventing cross-contamination and reducing biohazard risks.
Capable of sorting up to 200 cells per second with high accuracy, the VERLO™ supports both bulk and single-cell sorting. The integrated Single-Cell Dispenser deposits 1 to 100 cells per well in a 96- or 384-well plate. With 488 nm and 561 nm lasers, it detects up to nine fluorescent markers and three label-free parameters, providing insights into cell structures, internalisation, morphology, and viability.
Users can gate on image features like Area vs Max Intensity, similar to traditional FSC vs BSC gating. Real-time image analysis allows sorting without prior full-sample analysis, optimising workflow efficiency.
- Disposable sorting cartridges for both bulk and single-cell sorting.
- Piezoacoustic actuator ensures gentle and precise sorting with instant accuracy feedback.
- Bulk sorting capability, directing two selected populations into separate channels.
- Single-cell deposition for precise plating in 96- or 384-well formats.
- CellVantage software, integrating flow cytometry tools with an interactive image gallery.
Consumables
| Microfluidic Sorting Cartridges | |
| NC-150501 | Singel Cell Sorting Cartridges, 3 pcs/box |
| NC-150502 | Singel Cell Sorting Cartridges, 10 pcs/box |
| NC-150511 | Bulk sorting cartridges, 3 pcs/box |
| NC-150512 | Bulk sorting cartridges 10 pcs/box |
| Calibration beads | |
| NC-170111 | Nanocellect Calibration beads |
| NC-170123 | Nanocellect Rainbow beads, 8 peak |
Downloads
VERLO Image Guided Cell Sorter Brochure
Technical data
| Fluidics | |
| Sample input | 1.5, 2.0, and 5.0 mL tubes |
| Sheath input | 50 mL conical tubes |
| Sheath fluid | Buffer of choice |
| Sheath fluid usage | 9.6 mL/hour |
| Sample flow rate | 12 µL/minute |
| Sample line volume | 50 µL |
| Minimum sample volume | 100 µL |
| Tubing diameter (inner) | 200 to 500 µm |
| Flow cell | 200 x 700 µm |
| Smaller channel diameter | 70 µm |
| Sample pressure | < 2 psi |
| Sample output (bulk sorting) | 1.5 mL or 5 mL tubes |
| Sample output (single cell) | 96- or 384-well plates (flat/U/V bottom or PCR) |
| Plate Sorting Specifications | |
| Time to plate (96 wells) | 6 minutes |
| Time to plate (384 wells) | 20 minutes |
| Droplet volume | 4 µL |
| Sample plate options | 96 or 34 well (flat bottom, V-bottom, U-bottom, PCR) |
| Performance | |
| Scatter sensitivity | < 1.5 µm by FSC or BSC |
| Scatter resolution | Resolves lymphocytes, monocytes and granulocytes |
| Fluorescence sensitivity | < 200 MESF FITC (488 nm laser)< 250 MESF PE (561 nm laser) |
| Fluorescence resolution | 9-peak separation with SPHERO Rainbow Calibration Particles |
| Analysis speed | 2,000 events/second |
| Sorting | 1- and 2-ways |
| Back-to-back sorting speed | 200 events/second |
| Absolute counts | Yes |
| Volumetric counts | Yes |
| Warm-up time | Less than 1 minute |
| Sorting purity | Up to 99% purity |
| Optics | |
| Laser Profile | 1.5 x 1.5 µm |
| Scatter detection | Forward (0 degrees, +/- 15) Back (180 degrees, +/- 15) |
| Excitation & emission detection | 488 mm 50 mW: 525/50, 580/25, 620/50, 706/95, 760LP 561nm 50 mW: 580/25, 620/50, 706/95, 760LP |
| Optical alignment | Fixed alignment No maintenance required |
| Instrument Specifications | |
| VERLO Dimensions | 18 in H x 40 in W x 18 in D (45.7 cm H x 101.6 cm W x 45.7 cm D) |
| VERLO weight | 150 lbs / 68 kg |
| VERLO Electrical | AC Input: 100-240V, 50-60Hz, 2A |
