Key features
- Compact, robust and portable format for benchtop use
- Sterile, disposable fluidics for rapid clean-up without sample carryover
- Two-way sorting by scattering and up to nine emission colours (2 lasers)
- Low pressure system (<2 psi)
- Easy-to-use software
Sales in
Austria, Belgium, Denmark, France, Germany, Ireland, Luxembourg, Sweden, The Netherlands
WOLF® G2 Cell Sorter – Gentle microfluidic cell sorting
The new WOLF® G2 instrument has significantly expanded the capabilities of gentle benchtop microfluidic cell sorting with two lasers and up to nine colors, while maintaining simple workflows for either bulk sorting or single-cell dispensing. Whether you are focused on biopharma, genomics, or basic research, NanoCellect® delivers sterile, portable, intuitive solutions for gently sorting samples. Three laser combinations are available: 488/405 nm, 488/561 nm and 488/637 nm. The NanoCellect® WOLF® G2 uses a patented, microfluidic-based technology with robust laser-detection and easy-to-use WOLFviewer software. This in combination with the N1 Single Cell Dispenser allows higher cell viability including the opportunity to use sorted cells for further experiments. Cell sorting made easy.
Consumables
| NC-170111 | Calibration Beads |
| NC-150411 | Cartridge single cell sort |
| NC-150410 | Cartridge bulk sort |
| NC-170150 | Rainbow Beads 9 peak |
Accessories
| NC-13020 | Chiller-Stirrer |
| Art.-Nr. | N1 Pro Single Cell Dispenser |
Downloads
Brochure
Technical data
Fluidics |
|
| Sample Input | 1.5 and 5.0 mL tubes |
| Sheath Input | 50 mL Conical Tubes |
| Sheath Fluid | PBS or Buffer of Choice |
| Sheath Fluid Usage | 7.5 mL/Hour |
| Sample Flow Rate | 24 ul/Minute |
| Sheath Flow Rate | 160 uL/Minute |
| Dead Volume | 50 uL |
| Minimum Sample Volume | 150 uL |
| Tubing Diameter (Inner) | 200 to 500 um |
| Flow Cell | 200 x 70 um |
| Sample Pressure | < 2psi |
Optics |
|
| Excitation | 488 nm & 637 nm or 488 nm & 561 nm or 405 nm & 488 nm |
| Laser Profile | 25 x 27 µm |
| Scatter Detection | Forward (0 Degrees, +/-15) ; Back (180 Degrees, +/-15) |
| Emission Detection | See WOLF G2 Configuration Guide |
| Optical Alignment | Fixed alignment, no customer maintenance required |
Performance |
|
| Scatter sensitivity | < 1.5 μm by FSC or BSC |
| Scatter Resolution | Resolves lymphocytes, monocytes, and granulocytes |
| Fluorescence sensitivity | < 200 MESF FITC (using 488 nm laser) < 250 MESF PE (using 561 nm laser) |
| Fluorescence Resolution | 8 peak separation with SPHERO™️ Rainbow Calibration Particles |
| Analysis Speed | > 5,000 events/second |
| Sorting | 1- and 2-way |
| Back-to-back Sorting Speed | 200 events per second |
| Absolute Counts | Yes |
| Volumetric Analysis | Yes |
| Warm-up Time | Less than 1 minute |
| Sorting Purity | Up to 99% purity |
| Sorting Output | 1.5 mL and 5.0 mL, 96- and 384-well plates |
Physical Specifications |
|
| WOLF Dimensions | 14.8 H x 18.0 W x 13.6 D in (37.6 H x 45.8 W x 34.5 D cm) |
| WOLF Weight | 54 lbs (24.5 kg) |
| WOLF Electrical | AC Input: 100-240 V, 50/60 Hz, 0.5 A |
| N1 Dimensions | 8.42 W x 6.5 H x 8.34 D in (21.4 x 16.5 x 20.9) |
| N1 Weight | 5.5 lbs / 2.5 kg |
| N1 Electrical | DC Input: 24 V 1A |
