I&L Biosystems is excited to introduce the VERLO™ Image-Guided Cell Sorter that combines flow cytometry, gentle microfluidic sorting, and imaging to sort cells by morphology and localisation. With two lasers and 12 parameters, it enables bulk and single-cell sorting.
The VERLO™ Image-Guided Cell Sorter by NanoCellect utilises patented microfluidic-based sorting, combining laser excitation with sensitive PMT detectors to isolate mammalian cells, microbes, and plant cells. A precise piezoelectric actuator directs cells into collection channels, enabling analysis and sorting in a disposable format, preventing cross-contamination and reducing biohazard risks.
Capable of sorting up to 200 cells per second with high accuracy, the VERLO™ supports both bulk and single-cell sorting. The integrated Single-Cell Dispenser deposits 1 to 100 cells per well in a 96- or 384-well plate. With 488 nm and 561 nm lasers, it detects up to nine fluorescent markers and three label-free parameters, providing insights into cell structures, internalisation, morphology, and viability.
Users can gate on image features like Area vs Max Intensity, similar to traditional FSC vs BSC gating. Real-time image analysis allows sorting without prior full-sample analysis, optimising workflow efficiency.
- Disposable sorting cartridges for both bulk and single-cell sorting.
- Piezoacoustic actuator ensures gentle and precise sorting with instant accuracy feedback.
- Bulk sorting capability, directing two selected populations into separate channels.
- Single-cell deposition for precise plating in 96- or 384-well formats.
- CellVantage software, integrating flow cytometry tools with an interactive image gallery.
Key features
- Captures Spatial Data, including cell morphology and marker localisation.
- Visualises Subcellular Structures, analysing spatial variations in brightfield, darkfield, and fluorescence images.
- Enhances Sorting Precision by integrating imaging features into gating strategies for accurate cell phenotype identification.
- Combines High-Throughput Sampling with Single-Cell Imaging, extracting extensive feature data from each cell.
