Opentrons® OT-2 Robot

Opentrons

Pipetting Robot for Lab Automation

The OT-2 robot is a compact liquid handler developed to enable affordable automation of many common lab applications. It is an open-source system that can automate a range of applications across genomics, proteomics and drug discovery workflows. The OT-2 can be used with any automation-compatible lab materials.

Large_Format_OT2-R_with_Thermocycler OT2-R_DOOR_OPEN OT2-R_HERO OT2-R_Front OT2-R OT2-R_REMOVABLE_DECK

Key features

  • Benchtop size
  • Different modules for your individual workflow or use pre designed workstations
  • Compatible to ANSI/SLAS standard lab materials
  • Easily reconfigured – Friendly Protocol Designer
  • Accurate pipettes with wide volume range (1-1000 µL)
  • Fast-fill a 96 well plate in 22 seconds

Sales in

Austria, Belgium, Denmark, France, Germany, Ireland, Luxembourg, Sweden, Switzerland, The Netherlands, United Kingdom

Opentrons® OT-2 pipetting robot – affordable and flexible lab automation

The Opentrons OT-2 benchtop pipetting robot is a flexible system designed to automate life science workflows that involve liquid handling. The instrument is based on a modular platform.

Lab automation presents several challenges, and most solutions are either expensive and complex or easy to use but limited to specific workflows. The OT-2 robot fills this gap, allowing you to choose from a wide range of modules and pipettes.

The Opentrons OT-2 robot will: 

    • Enable increased throughput
    • Reduce hands-on time
    • Improve consistency across samples and runs
    • Help optimize time-consuming protocols
    • Provide highly accurate results

The list of applications for the Opentrons OT-2 liquid handler is endless. Commonly used applications are: 

    • Nucleic Acid Extraction
    • PCR
    • ELISA
    • MGS Library preparation
    • Reagent of sample aliquoting
    • Serial dilutions
    • Biochemical assays
    • Small-scale protein purification
    • LC-MS sample preparation

In addition, the OT-2 is compatible with ANSI/SLAS-compliant labware and automation-friendly consumables, so you can keep using your own labware.

Workstations

The OT-2 is a flexible and easy-to-implement system for many different types of laboratories. Choose from the list of pre-designed workstations covering the most popular applications, or build your own system with the on-deck hardware modules and swappable 1- and 8-channel pipettes.

Workstations include the OT-2 robot, modules, pipettes, and all necessary labware to accommodate your selected application. All workstations can be customized by adding or removing available hardware modules to suit your workflow. For more options and additional information, please get in touch with your local sales representative.

Available workstations are: 

  • PCR workstation for PCR setup and thermocycling (max 96 samples)
  • Cell assay-based workstation
  • Pooling and normalization workstation
  • Dual flow chromatography protein purification workstation (max 96 samples)
  • Sample aliquoting workstation (max 96 samples)

Available on-deck hardware modules: 

  • Temperature Module (GEN2)
  • HEPA Module (GEN1)
  • Magnetic Module (GEN2)
  • Heater-Shaker Module
  • Thermocycler Module
  • Pipettes

Software and protocols

The Opentrons app controls the robot and offers flexible options for creating individual protocols. The protocol software is adaptable to various workflows and does not require coding knowledge. Beginners can either create a protocol directly in the software or load established protocols from the library, while experienced programmers can use the open-source software Python API.

Opentrons OT-2 versus the Opentrons Flex™ liquid handler

Are you in doubt about which robot will suit your needs best?

OT-2 

  • Ideal for sample reformatting, reagent preparation, and routine workflows
  • Fits in a biological safety cabinet
  • Run sensitive contamination-prone applications by using the HEPA Filter module

Opentrons Flex: 

  • Ideal for higher throughput and more complex workflows
  • Maximizes walk-away time
  • 1-, 8-, and 96-channel pipetting
  • Integrated gripper – can move labware to and from slots on the deck
  • Deck expander – gives you 4 additional deck slots for tips and labware

Downloads

Brochure

Quick start guide

User manual

Safety and regulatory compliance information

Performance graphs

Preventive maintenance guide

Technical data

Dimensions (WxDxH) 63 × 57 × 66 cm
Weight 48 kg
Pipette overview Smart sensors support automatic calibration, real-time positioning, and error detection
Pipette volumes and specs 1-Channel Pipettes: 1–1000 µL

8-Channel Pipettes: 1–300 µL,

Deck 11 main ANSI/SLAS-format deck slots

Deck can be reconfigured by removing deck slots and replacing them with other deck features

Connectivity WiFi 2,4 GHz IEEE 802.11b/g/n, USB 2.0
Video Integrated video camera (included for future or custom functionality)
Audio Integrated audio system (Included for future or custom functionality)
Power requirements Robot Power Input: 36 VDC, 6.1 A

Power Adapter Input: 100–240 VAC, 50/60 Hz, 4.0 A/115 VAC, 2.0 A/230 VAC

Frame composition Rigid steel and CNC aluminum design
Operating temperature & relative humidity Temperature: Recommended 20–25 °C

Humidity: Recommended 40–60% RH

1-channel accuracy & precision 15% accuracy at 1 μL

5% precision at 1 μL

Sterility Full polycarbonate enclosure designed to limit exposure. The OT-2 is not a sterile environment. Add the Opentrons HEPA Module, or add your own UV lights
API compatibility Opentrons software is open-source; integration of other software to interface is possible by the customer
Interface Windows 10 or later, macOS 10.10 or later, Ubuntu 12.04 or later.

Opentrons App available

Tip compatibility Use Opentrons Flex Tips and Opentrons Flex Filter Tips

Third party tip performance cannot be guaranteed by Opentrons.

Labware compatibility Use common labware already in our Labware Library, or add your own.
Warranty One year warranty against manufacturer defects
Certifications Certifications complete: CE, UL, FCC, ISO 9001