The SuperFlo® column consists of two concentric porous cylinders, creating a space. This area between the frits is the column bed and this is packed with the chromatographic resin. The eluent/sample enters at the top of the column, the top lid acts as a distribution head and directs the sample into the gap between the outer column body and the outer frit. It distributes over the whole height of the column and flows horizontal from the outer frit to the inner core. From the gap between the inner frit and the collector rod (spacer in the centre of the column) the eluent leaves through the outlet at the bottom of the column. The effective bed height of the column is the distance between inner and outer frit.

The intrinsic design of the SuperFlo® column has a significantly larger surface area than a tradtional  axial column, therefore even at high flow rates the linear velocity is in a range which allows for the required interaction between sample and resin. SuperFlo® allows to make your separation faster and in certain cases you can save expensive resin (e.g. Protein G) because you can make 2 runs in the same time – means you can reduce the size of the column by 50%. The investment into a SuperFlo® column will be compensated by the savings in resin costs after the first column packing.